Journal: Cell

Article Title: Hijacking oogenesis enables massive propagation of LINE and retroviral transposons

doi: 10.1016/j.cell.2018.06.040

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Aub Senti et al. , 2015 N/A Mouse monoclonal anti-Ago3 Senti et al. , 2015 N/A Mouse monoclonal anti-γH2Av DSHB CAT#: UNC93–5.2.1 Rabbit polyclonal anti-Vasa Lasko et al. , 1990 N/A Mouse anti-Gurken DSHB CAT#: 1D12-s Alexa 488 Donkey anti-Mouse Molecular Probes CAT#: A21202 Alexa 594 Goat anti-Rabbit Molecular Probes CAT#: A11037 Experimental Models: Organisms/Strains D.melanogaste: w 1118 Brennecke et al. , 2008 N/A D.melanogaster: w k Brennecke et al. , 2008 N/A D.melanogaster: P(otu-Gal4::VP16.R)1 w[*]; P(Gal4-nos.NGT)40; P(Gal4::VP16-nos.UTR)CG6325[MVD1] Bloomington Drosophila Stock Center CAT#: 31777 D.melanogaster: white -sh (attP2) Senti et al. , 2015 N/A D.melanogaster: aub -sh (attP40) and ago3-sh (attP2) Senti et al. , 2015 N/A D.melanogaster: X-Y attached male: c(1;Y)1,yw/O Robert Levis (Spradling lab) N/A D.melanogaster: w 1118 -I-element -eGFP - reporter This paper N/A Competent cells Stbl2 ThermoFisher CAT#: 10268019 Stbl4 ThermoFisher CAT#: 11635018 Critical Commercial Assay Nextera DNA Library Prep Illumina CAT#: FC-121–1030 and FC121–1011 Quick-gDNA MicroPrep Zymo Research CAT#: D3021 DNA Clean and Concentrator-5 Zymo Research CAT#: D4004 FISH Wash Buffer A LGC Biosearch Tech CAT#: SMF-WA1–60 FISH Hybridization Buffer LGC Biosearch Tech CAT#: SMF- HB1–10 FISH Wash Buffer B LGC Biosearch Tech CAT#: SMF- WB1–20 Vectashield Mounting Medium VECTOR LABORATORIES INC MS CAT#: 101098–042 Demecolcine Sigma CAT#: D7385 Deposited Data Illumina Sequencing raw data (FASTQ) This paper https://www.ncbi.nlm.nih.qov/bioproiect/PRJNA454868 RNA sequencing data Senti et al. , 2015 GSE71775 Oligonucleotides Forward primer to amplify I-element without 3′UTR: CCG GAA TTC TTA GTA AAT TAT TAA TAT GCA AAT CAT TAC CAC TTC AAC CTC CG AAG This paper N/A Reverse primer to amplify I-element without 3′UTR: TTT TCC TTT TGC GGC CGC ACA TGC ATG CAT GTC GAC GCG TCG TTG GCG CGC CAC AAA TGC CTG TTT TGT TTT TAG A This paper N/A Forward primer to amplify the eGFP-reporter-I-3′UT: CGT TGG CGC GCC GGT ACC GAA T This paper N/A Reverse Primer to amplify the eGFP-reporter-I-3′UTR: CGA TAC ATG CAT GCA AAA CGT CTT TGC CAC GAT T This paper N/A Software and Algorithms piPipes Han et al. , 2015 N/A TEMP Zhuang et al. , 2014 N/A circlcize Gu et al. , 2014 N/A Other Open in a separate window KEY RESOURCES TABLE

Techniques: Hybridization, Plasmid Preparation, Sequencing, RNA Sequencing Assay, Software

Journal: Cell

Article Title: Hijacking oogenesis enables massive propagation of LINE and retroviral transposons

doi: 10.1016/j.cell.2018.06.040

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Aub Senti et al. , 2015 N/A Mouse monoclonal anti-Ago3 Senti et al. , 2015 N/A Mouse monoclonal anti-γH2Av DSHB CAT#: UNC93–5.2.1 Rabbit polyclonal anti-Vasa Lasko et al. , 1990 N/A Mouse anti-Gurken DSHB CAT#: 1D12-s Alexa 488 Donkey anti-Mouse Molecular Probes CAT#: A21202 Alexa 594 Goat anti-Rabbit Molecular Probes CAT#: A11037 Experimental Models: Organisms/Strains D.melanogaste: w 1118 Brennecke et al. , 2008 N/A D.melanogaster: w k Brennecke et al. , 2008 N/A D.melanogaster: P(otu-Gal4::VP16.R)1 w[*]; P(Gal4-nos.NGT)40; P(Gal4::VP16-nos.UTR)CG6325[MVD1] Bloomington Drosophila Stock Center CAT#: 31777 D.melanogaster: white -sh (attP2) Senti et al. , 2015 N/A D.melanogaster: aub -sh (attP40) and ago3-sh (attP2) Senti et al. , 2015 N/A D.melanogaster: X-Y attached male: c(1;Y)1,yw/O Robert Levis (Spradling lab) N/A D.melanogaster: w 1118 -I-element -eGFP - reporter This paper N/A Competent cells Stbl2 ThermoFisher CAT#: 10268019 Stbl4 ThermoFisher CAT#: 11635018 Critical Commercial Assay Nextera DNA Library Prep Illumina CAT#: FC-121–1030 and FC121–1011 Quick-gDNA MicroPrep Zymo Research CAT#: D3021 DNA Clean and Concentrator-5 Zymo Research CAT#: D4004 FISH Wash Buffer A LGC Biosearch Tech CAT#: SMF-WA1–60 FISH Hybridization Buffer LGC Biosearch Tech CAT#: SMF- HB1–10 FISH Wash Buffer B LGC Biosearch Tech CAT#: SMF- WB1–20 Vectashield Mounting Medium VECTOR LABORATORIES INC MS CAT#: 101098–042 Demecolcine Sigma CAT#: D7385 Deposited Data Illumina Sequencing raw data (FASTQ) This paper https://www.ncbi.nlm.nih.qov/bioproiect/PRJNA454868 RNA sequencing data Senti et al. , 2015 GSE71775 Oligonucleotides Forward primer to amplify I-element without 3′UTR: CCG GAA TTC TTA GTA AAT TAT TAA TAT GCA AAT CAT TAC CAC TTC AAC CTC CG AAG This paper N/A Reverse primer to amplify I-element without 3′UTR: TTT TCC TTT TGC GGC CGC ACA TGC ATG CAT GTC GAC GCG TCG TTG GCG CGC CAC AAA TGC CTG TTT TGT TTT TAG A This paper N/A Forward primer to amplify the eGFP-reporter-I-3′UT: CGT TGG CGC GCC GGT ACC GAA T This paper N/A Reverse Primer to amplify the eGFP-reporter-I-3′UTR: CGA TAC ATG CAT GCA AAA CGT CTT TGC CAC GAT T This paper N/A Software and Algorithms piPipes Han et al. , 2015 N/A TEMP Zhuang et al. , 2014 N/A circlcize Gu et al. , 2014 N/A Other Open in a separate window KEY RESOURCES TABLE

Techniques: Hybridization, Plasmid Preparation, Illumina Sequencing, RNA Sequencing, Software